The novel role of DUSP4 in suppressing ferroptosis and promoting cytotoxicity of CD8+ T cells in MSI colorectal cancer
Zhang Dongsheng 1, Yang Sheng 1,2, Xu Hengjie 1,2, Chen Zhihao 1,2, Wang Xiaowei 1,2, Sun Yueming 1,3,4,5
1 https://ror.org/04py1g812 Department of General Surgery, Colorectal Institute of Nanjing Medical University The First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu China
2 https://ror.org/059gcgy73 The First School of Clinical Medicine Nanjing Medical University Nanjing Jiangsu China
3 Jiangsu Province Engineering Research Center of Colorectal Cancer Precision Medicine and Translational Medicine Nanjing Jiangsu China
4 https://ror.org/059gcgy73 Collaborative Innovation Center for Cancer Personalized Medicine Nanjing Medical University Nanjing Jiangsu China
5 https://ror.org/04fe7hy80 The Affiliated Shuyang Hospital of Xuzhou Medical University Suqian Jiangsu China
Abstract
Background Microsatellite instable (MSI) colorectal cancer (CRC) has distinct features that distinguish it from microsatellite stable CRC. While ferroptosis may play a role in the development of MSI CRC, its mechanisms remain unclear.
Methods Ferroptosis was assessed via the detection of lipid peroxidation, malondialdehyde, 4-hydroxy-2-nonenal, and intracellular Fe2+, etc. Phosphoproteomic analysis, cytokine array, and flow cytometry were performed...
